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Updated: Aug 21, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
[Methods of enumeration of bacteria in drinking water]
Wei Lu1, Yun Wang, Xiao-jian Zhang
1Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Abstract:
Methods of the enumeration of total bacteria and Coliform in drinking water were researched in this paper. The differences between heterotrophic plate count and direct viable count method were compared. It is concluded that the total number of bacteria in R2A medium is one order of quantity higher than the traditional plate count agar, and the results of acridine orange direct counts (AODC) is the highest. Some different staining methods in direct viable count were also compared in this paper, such as nalidixic acid, CTC staining and BacLight staining. The proportion of the live bacteria to the dead is about 10%.
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