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Flow injection-chemiluminescence determination of puerarin in pharmaceutical preparations
Qunlin Zhang1, Aung Myint, Lijuan Liu
1Department of Chemistry, University of Science and Technology of China, Jinzai Road 96, Hefei, Anhui 230026, PR China.
Abstract:
Strong chemiluminescence was observed when cerium(IV) reacted with rhodamine 6G in sulfuric acid medium in the presence of puerarin. This phenomenon has been utilized to design a sensitive and selective flow injection-chemiluminescence method for the determination of puerarin. Under the optimum conditions, the proposed procedure has a linear range between 1.3 x 10(-9) and 8.0 x 10(-7)g/mL, with a detection limit of 8.4 x 10(-10)g/mL puerarin and a relative standard deviation of 1.86% (n = 11) at 5.0 x 10(-8)g/mL puerarin. The method was successfully applied to the determination of puerarin in pharmaceutical preparations. The mechanism of this chemiluminescence reaction has been proposed.
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