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08:44
Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
Published on: October 23, 2014
Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex
Zophonías O Jónsson1, Sudhakar Jha, James A Wohlschlegel
1University of Virginia School of Medicine, Jordan 1240, 1300 Jefferson Park Avenue, Charlottesville, VA 22908, USA.
Molecular Cell
|November 5, 2004
Summary
The essential nuclear ATP binding proteins Rvb1p and Rvb2p are crucial for the structural integrity of the Ino80 chromatin remodeling complex. Their absence disrupts catalytic activity and leads to the loss of critical components like Arp5p.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- Rvb1p and Rvb2p (TIP48/49) are conserved nuclear ATP-binding proteins essential for eukaryotic cell viability.
- These proteins form a double hexamer and associate with chromatin remodeling complexes, including Ino80.com and Swr1.com.
Purpose of the Study:
- To investigate the role of Rvb1p and Rvb2p in the function and integrity of the Ino80 chromatin remodeling complex.
- To determine the necessity of Rvbp for Ino80p recruitment and catalytic activity.
Main Methods:
- Yeast genetics to study the effects of Rvb1p/Rvb2p and Ino80p loss on gene promoters.
- In vitro biochemical assays to examine Rvb2p association with Arp5p and Ino80p.
Main Results:
- Loss of Rvb1p or Ino80p impacts numerous yeast promoters similarly.
- Rvbp are not required for Ino80p recruitment but are essential for Ino80.com's catalytic activity.
- Loss of Rvbp results in the loss of Arp5p from the Ino80.com, and Rvb2p associates with Arp5p in an ATP- and Ino80p-dependent manner.
Conclusions:
- Rvbp are indispensable for maintaining the structural and functional integrity of the Ino80 chromatin remodeling complex.
- The findings highlight the critical role of Rvbp in chromatin remodeling processes essential for cell viability.
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