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Effect of Pseudomonas aeruginosa (MTCC 1688) on the tissue phosphatases activity on Macrobrachium rosenbergii (De
1PG & Research Department of Zoology, Govt. Arts College, Nandanam, Chennai-600 035, India. sunilnioh@yahoo.com
Abstract:
A time course study on the endotoxin toxicity of the gram negative bacteria, Pseudomonas aeruginosa MTCC 1688 on the tissue phosphatases activity on the giant freshwater prawn, Macrobrachium rosenbergii was conducted. The results revealed marked elevation of both acid and alkaline phosphatase activity in the haemolymph and body muscle. The hepatopancreas showed reduced phosphatase activity compared to control. The enzymes, being non-specific in action and particularly the acid phosphatase being of lysosomal origin, their increase in muscle and haemolymph has pathogenic significance in the inoculum treated prawns.
Insights
Pseudomonas aeruginosa endotoxin exposure increased phosphatases in Macrobrachium rosenbergii. Acid and alkaline phosphatase activity rose in prawn hemolymph and muscle, indicating pathogenic effects.
Area of Science:
- Environmental toxicology
- Aquatic animal health
- Microbial pathogenesis
Background:
- Pseudomonas aeruginosa is a gram-negative bacterium known for its endotoxin production.
- Endotoxins can induce significant physiological stress and damage in aquatic organisms.
- Tissue phosphatases are key enzymes involved in various metabolic processes and can indicate cellular damage.
Purpose of the Study:
- To investigate the time-course effects of Pseudomonas aeruginosa endotoxin on tissue phosphatases activity.
- To assess the impact of endotoxin toxicity on the giant freshwater prawn, Macrobrachium rosenbergii.
Main Methods:
- A time-course study was conducted exposing Macrobrachium rosenbergii to Pseudomonas aeruginosa MTCC 1688 endotoxin.
- Assays were performed to measure acid and alkaline phosphatase activity in hemolymph, body muscle, and hepatopancreas.
Main Results:
- A marked elevation in both acid and alkaline phosphatase activity was observed in the hemolymph and body muscle of treated prawns.
- Conversely, phosphatase activity in the hepatopancreas was reduced compared to control groups.
- The observed increases in hemolymph and muscle phosphatase activity suggest pathogenic implications.
Conclusions:
- Pseudomonas aeruginosa endotoxin significantly alters tissue phosphatase activity in Macrobrachium rosenbergii.
- Elevated acid and alkaline phosphatase levels in hemolymph and muscle indicate cellular damage and stress.
- The findings highlight the pathogenic significance of endotoxin exposure in prawns.
