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Related Experiment Videos

The case for nuclear translation.

Francisco J Iborra1, Dean A Jackson, Peter R Cook

  • 1MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DS, UK.

Journal of Cell Science
|November 13, 2004
PubMed
Summary
This summary is machine-generated.

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Recent studies indicate that translation, the process of protein synthesis, may occur within eukaryotic cell nuclei, coupled with transcription. This nuclear translation is supported by evidence of nonsense-mediated decay (NMD) and direct observation of polypeptide synthesis in the nucleus.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Traditionally, eukaryotic translation is considered a cytoplasmic process.
  • Recent findings challenge this dogma, suggesting nuclear translation may occur.
  • This process is hypothesized to be coupled with transcription.

Purpose of the Study:

  • To review and present evidence supporting the occurrence of nuclear translation in eukaryotes.
  • To explore the functional implications of nuclear translation, particularly its link to transcription and mRNA quality control.

Main Methods:

  • Analysis of existing research on nuclear mRNA decay pathways, specifically nonsense-mediated decay (NMD).
  • Review of studies demonstrating amino acid incorporation into nuclear polypeptides.

Related Experiment Videos

  • Examination of co-localization and co-immunoprecipitation data for translation, NMD, and transcription factors.

    • Main Results:

    • Nonsense-mediated decay (NMD) occurring in the nuclear fraction suggests active nuclear ribosomes.
    • Direct evidence shows tagged amino acids incorporated into nascent polypeptides within the nucleus during transcription.
    • Components of translation, NMD, and transcription machinery co-localize and interact within the nucleus.

    Conclusions:

    • The evidence collectively supports the occurrence of bona fide nuclear translation.
    • Nuclear translation is likely coupled to transcription, potentially aiding in mRNA surveillance.
    • Alternative explanations involving cytoplasmic ribosome contamination are less likely given the integrated evidence.