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Related Experiment Videos

In vitro activation of human complement by polyacrilonitrile dialyzer membrane assayed by complement binding to

K A Shastri1, G L Logue

  • 1Department of Medicine, State University of New York, Buffalo.

ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|January 1, 1992
PubMed
Summary

Polyacrylonitrile (PAN) dialyzer membranes activate complement more than Cuprophan. This study used C3d deposition on red blood cells to accurately measure complement activation by dialyzer membranes.

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Area of Science:

  • Biomaterials Science
  • Immunology
  • Nephrology

Background:

  • Quantifying complement activation by polyacrylonitrile (PAN) dialyzer membranes is challenging due to their high adsorption of anaphylotoxins.
  • Standard assays may underestimate complement activation by PAN membranes.

Purpose of the Study:

  • To explore alternative methods for measuring in vitro complement activation by PAN and Cuprophan membranes.
  • To compare the complement activation potential of PAN and Cuprophan membranes.

Main Methods:

  • Incubating normal human erythrocytes with PAN and Cuprophan membranes in serum.
  • Measuring C3d deposition on erythrocytes as an indicator of complement activation.

Main Results:

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  • PAN membranes induced significantly higher C3d deposition on erythrocytes compared to Cuprophan membranes.
  • This indicates greater complement activation by PAN membranes.

    • Conclusions:

    • The C3d deposition assay provides a reliable method for assessing complement activation by dialyzer membranes.
    • PAN membranes exhibit a higher potential for complement activation than Cuprophan membranes, warranting further investigation into the clinical implications.