Related Experiment Video

Updated: Aug 20, 2026

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice
07:25

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Published on: September 25, 2019

[Suppression of c-myc expression by interference RNA in HepG2 hepatocellular carcinoma cells]

Yang Xu1, Yi-Hua Wang, Ji-Dong Gao

  • 1Department of Molecular Immunology, Cancer Institute (Hospital), Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100021, China.

Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology]
|November 24, 2004
PubMed
Abstract

Insights

RNA interference targeting the c-myc gene significantly inhibited its expression in hepatocellular carcinoma cells (HepG2). This suppression of c-myc may induce cancer cell apoptosis.

Area of Science:

  • Molecular biology
  • Cancer research
  • Gene silencing

Context:

  • Hepatocellular carcinoma (HCC) is a significant global health concern.
  • The c-myc oncogene plays a crucial role in HCC development and progression.
  • Targeting oncogenic pathways is a key strategy in cancer therapy.

Purpose:

  • To investigate the efficacy of RNA interference (RNAi) in suppressing c-myc expression in HepG2 cells.
  • To evaluate the impact of c-myc inhibition on hepatocellular carcinoma cell apoptosis.

Summary:

  • A c-myc gene-targeting small interfering RNA (siRNA) expression vector (psilencer-c-myc) was constructed and transfected into HepG2 cells.
  • Quantitative PCR and Western blot confirmed significant suppression of c-myc mRNA and protein levels (67% inhibition).
  • Flow cytometry and immunofluorescent microscopy demonstrated increased apoptosis in transfected cells.

Impact:

  • Demonstrates successful inhibition of c-myc at both transcriptional and translational levels in HCC cells using siRNA.
  • Suggests a potential therapeutic strategy for hepatocellular carcinoma by targeting c-myc and inducing apoptosis.

More Related Videos

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells11:11

Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

Published on: April 25, 2018

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...