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Updated: Aug 20, 2026
Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction
Published on: May 28, 2012
[Study on mimotopes of E.coli lipopolysaccharide 2630]
Hai-bo Luo1, Shan-gen Zheng, Ping Zhu
1Department of Immunology, First Military Medical University, Guangzhou 510515, China. seaman@fimmu.com
Aim:
To screen mimotopes of E.coli lipopolysaccharide(LPS) 2630 from c7c phage display peptide library.
Methods:
The LPS mimotopes were screened from c7c phage display peptide library by using affinity chromatograph-purified polyclonal antibody against E.coli LPS 2630(L2630), and the antigenicity of selected clones was identified by ELISA.
Results:
After 3 rounds of biopanning, 12 out of 20 phage clones were identified as positive clones which could bind to polyclonal anti-L2630 antibody, and 5 of these 12 clones could bind to polyclonal anti-S.typhi LPS 7261(L7261) antibody. The deduced amino acid sequence analysis showed that 8 of 12 clones had the conservative sequence: X-DGLL-XX or X-EDGLL-X.
Conclusion:
The peptides displayed on these phage clones can mimic the epitopes of L2630, and 5 of these phage clones mimic the common epitopes of L2630 and L7261.
