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Updated: Aug 20, 2026
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
[Construction and identification of anti-chymopapain scFv phage display library]
Jian-ping Wang1, Shu-jin Zhao, Jie Wang
1Department of Bioengineering, South China University of Technology, Guangzhou 510640, China. bestwjp@sohu.com
Aim:
To construct phage display library of anti-chymopapain scFv.
Methods:
V(H) and V(L) gene repertoires were amplified from splenocyte mRNA by RT-PCR and joined by a (Gly(4)ser)3 linker to obtain scFv genes. The scFv genes were then cloned into phagemid pFAB5C to construct phage display library. Affinity selection and ELISA were used to identify specific phage antibody to chymopapain.
Results:
After 4 rounds of panning, high affinity scFv was obtained.
Conclusion:
Phage display library of anti-chymopapain scFv was successfully constructed, and scFv with binding ability to chymopapain was obtained.
