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Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
Published on: November 24, 2010
The influence of the non-nucleotide insert on the hybridization properties of oligonucleotides
Inna A Pyshnaya1, Dmitrii V Pyshnyi, Alexander A Lomzov
1Institute of Chemical Biology and Fundamental Medicine SD RAS, Novosibirsk, Russia. pyshnaya@niboch.ncs.ru
Abstract:
The effect of different non-nucleotide inserts incorporated into oligonucleotide chains on their hybridization properties was studied by the method of thermal denaturation. Various types of alkyldiols and oligoethylene glycols were used as inserts modifying oligonucleotide backbone. Such modification of oligonucleotides caused the destabilization of their complementary complexes. It was shown that the hybridization properties of the modified oligonucleotides depend on several features of inserts: the type, number, length of insertions, and positions of interrupted dinucleotide steps in oligonucleotide chain.
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