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Updated: Aug 20, 2026
Biochemical Measurement of Neonatal Hypoxia
Published on: August 24, 2011
[Determination of hypoxanthine and xanthine in hippocampus by HPLC method]
Xiaoli Zhao1, Xiaobing Cui, Liuqing Di
1Nanjing University of Traditional Chinese Medicine, Nanjing 210029.
Objective:
A method was established for the determination of hypoxanthine and xanthine in Hippocampus by HPLC.
Methods:
The HPLC system consisted of a Kromasil C18 column (250 mm x 4.6 mm, 5 microm) with methanol-water (2:98, V/V) as the mobile phase. The wavelength of detection was 254 nm.
Results:
The linear range of hypoxanthine and xanthine were 29.0-464.0 ng (r = 0.99996) and 24.5-392.0 ng (r = 0.9995) respectively. The average recovery of hypoxanthine was 100.73%, RSD = 1.31% (n = 6). The average recovery of xanthine was 100.54%, RSD = 1.14% (n = 6).
Conclusion:
This method is convenient and the result is accurate. It can be used for quality control of Hippocampus.
