Related Experiment Video

Updated: Aug 20, 2026

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)
07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

Published on: May 23, 2014

Photon antibunching proves emission from a single subunit in the autofluorescent protein DsRed

Gabriel Sánchez-Mosteiro1, Majolein Koopman, Erik M H P van Dijk

  • 1Applied Optics Group, Faculty of Science & Technology and MESA+, Institute for Nanotechnology, University of Twente, P O. Box 217, 7500 AE Enschede, Netherlands.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|December 8, 2004
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...