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Related Experiment Videos

A single cell electroporation chip.

Michelle Khine1, Adrian Lau, Cristian Ionescu-Zanetti

  • 1Berkeley Sensor & Actuator Center, Department of Bioengineering, University of California, Berkeley, CA 94720, USA.

Lab on a Chip
|December 24, 2004
PubMed
Summary
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Researchers developed a novel polymeric chip for efficient single-cell electroporation, enabling targeted delivery of polar substances into cells. This technology simplifies cell membrane permeabilization for research and therapeutic applications.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioengineering

Background:

  • Cell membrane permeability limits the introduction of essential polar molecules like drugs and DNA into cells.
  • Electroporation is a method to increase cell membrane permeability, but traditional techniques are complex and difficult to scale.
  • Single-cell manipulation is crucial for precise biological studies and targeted therapies.

Purpose of the Study:

  • To develop and validate a novel polymeric chip for efficient, localized, and parallel single-cell electroporation.
  • To demonstrate the chip's capability in introducing polar substances into single cells.
  • To determine the optimal electrical parameters for electroporating specific cell types.

Main Methods:

  • Development of a polymeric microfluidic chip for selective cell immobilization and electric field focusing.

Related Experiment Videos

  • Application of low-voltage (< 1 V) electroporation to single HeLa cells.
  • Electrical assessment of membrane permeation via current-voltage measurements.
  • Microscopic verification of membrane permeation using fluorescent dyes (Calcein AM and Trypan blue).

    • Main Results:

    • The polymeric chip successfully immobilized and electroporated single HeLa cells with low applied voltages.
    • The average transmembrane potential required for HeLa cell electroporation was determined to be 0.51 ± 0.13 V.
    • Electrical and microscopic analyses confirmed successful membrane permeation and substance delivery.

    Conclusions:

    • The developed polymeric chip offers a user-friendly and effective platform for parallel single-cell electroporation.
    • This technology facilitates the introduction of polar molecules into cells, with broad applications in cell biology and drug delivery.
    • The chip design simplifies electroporation procedures, reducing the need for complex electrode manipulation.