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Updated: Aug 20, 2026
Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
Published on: May 31, 2018
[Coxsackievirus B3-induction of tumor necrosis factor and IL-6 of murine monocyte-macrophages]
Abstract:
This study is to observe the infection of coxsackievirus B3 (Cox B3) to murine monocyte-macrophages (Mo/Mpsi), and its induction of tumor necrosis factor (TNF) and IL-6. Cox B3 was capable of infecting murine macrophages as revealed by immunofluorescence and releasing infectious virus particles. Virus infection with < or = 100 TCID50 did not reduce macrophages viability. Cox B3 stimulated the release of monokines from Mo/Mpsi in vitro and in vivo, markedly increasing the concentration of TNF and IL-6. These were found in the plasma and homogenized heart tissue during the acute phase of murine myocarditis induced by Cox B3, and in the culture supernatant of Mo/Mpsi infected by Cox B3.
Insights
Coxsackievirus B3 (Cox B3) infects murine macrophages, increasing tumor necrosis factor (TNF) and IL-6. These inflammatory cytokines are detected during Cox B3-induced myocarditis, indicating their role in the disease.
Area of Science:
- Virology
- Immunology
- Pathology
Context:
- Murine monocyte-macrophages (Mo/Mpsi) are key immune cells.
- Coxsackievirus B3 (Cox B3) is a significant cause of viral myocarditis.
- Understanding virus-host interactions is crucial for disease management.
Purpose:
- To investigate Coxsackievirus B3 infection in murine macrophages.
- To determine the induction of tumor necrosis factor (TNF) and IL-6 by Cox B3.
- To correlate cytokine levels with experimental murine myocarditis.
Summary:
- Coxsackievirus B3 effectively infects murine macrophages, confirmed by immunofluorescence and infectious virus release.
- Macrophages infected with Cox B3 showed no significant viability loss at doses <= 100 TCID50.
- Cox B3 stimulated the release of TNF and IL-6 from macrophages in vitro and in vivo, with elevated levels in plasma and heart tissue during acute myocarditis.
Impact:
- Demonstrates Cox B3's ability to infect and activate macrophages.
- Highlights the role of TNF and IL-6 in Cox B3-induced myocarditis.
- Provides insights into the inflammatory mechanisms underlying viral heart disease.
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