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Updated: Aug 20, 2026
13:04
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[In vitro infection of the human T-cell line with the hepatitis C virus]
Abstract:
A human T-cell line (Molt-4) was infected with an hepatitis C virus RNA (HCV- RNA) positive serum for 12 hours and incubated continously for 1-28 days. A productive infection was demonstrated by polymerase chain reaction amplification. Viral plus-stranded RNA was detectable in Molt-4 on the 1st, 2nd, 3rd day and the 8th day post infection. Viral minus-stranded RNA was detectable on the 2nd day and 8th day post infection.
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