Effect of iron absorption on plasma membrane proteins of small intestinal mucosal cells from iron-deficient rats
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Updated: Aug 20, 2026

The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
Published on: April 28, 2022
N Kaufman1, M Newkirk, J C Wyllie
1Department of Pathology, Queen's University and Kingston General Hospital, Kingston, Ontario, Canada.
Abstract:
Plasma membrane protein was isolated from small intestinal mucosal cells of normal and iron-deficient rats during iron absorption and examined by acrylamide gel electrophoresis. Two proteins were present in increased amounts in the iron-deficient animals 10 min after administration of iron; uptake studies with 3H-leucine indicated that each had been newly-synthesized. The molecular weights of the proteins were about 60,000 and 300,000.
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