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Updated: Aug 20, 2026

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
Published on: April 23, 2017
[Apoptosis-inducing ligand TRAIL can be recruited to lipid rafts]
1Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.
Aim:
To investigate the relationship between tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) and cell membrane microdomain lipid rafts.
Methods:
The expression of TRAIL on K562 cells was detected by indirect immunofluorescence staining and flow cytometry. The lipid rafts on K562 cells were detected with FITC-labeled cholera toxin B subunit (CTx-FITC) which bound to the GM1 glycosphingolipid in lipid rafts. The lipid rafts were then cross-linked with an anti-FITC mAb after binding with CTx-FITC. The localization of TRAIL on K562 cells was analyzed with rabbit anti-TRAIL antibody, Cy3-labeled goat-anti-rabbit IgG (Cy3-labeled GAR) and laser scanning confocal microscope.
Results:
The cross-linking of lipid rafts appeared at 20 minutes, reached peak at 30 minutes, and weakened around 40 minutes. After the cells were labeled with CTx-FITC and anti-FITC mAb TRAIL was aggregated in the cross-linked lipid rafts.
Conclusion:
The anti-FITC mAb could be used for cross-linking of lipid rafts. TRAIL can be recruited to lipid rafts.
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