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[Construction of an eukaryotic expression plasmid for short peptides used in DNA immunization]
Huan-Bin Xu1, Wei Xu, Yi-Wei Chu
1Department of Immunology, Shanghai Medical College of Fudan University, Shanghai 200032, China.
Aim:
To construct a plasmid which efficiently express short peptides in DNA immunization.
Methods:
The plasmid containing peptide-expressing cassette (PEC) was constructed and its effect in DNA immunization was investigated, using a DHBV B cell epitope as the short peptide. The peptide in vitro was detected by DOT-EIA. The BALB/c mice were immunized with the empty plasmid or the recombinant plasmid, and the specific antibodies against the epitope in the sera of the mice were determined by ELISA.
Results:
The plasmid containing peptide-expressing cassette (PEC) was successfully constructed. Recombinant epitope-based plasmid could efficiently express the short peptide in vitro and induce immune response against it in DNA immunization.
Conclusion:
The constructed vector provides highly efficient short peptide expression in DNA immunization.
