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Published on: November 23, 2016
Microcutting culture and morpho-physiological changes during acclimation in two Lycium chilense cytotypes
Pablo Horacio Maseda1, Jorge Hugo Lemcoff, Mercedes Murúa
1IFEVA--Cátedra de Fisiología Vegetal (CONICET-FAUBA), Universidad de Buenos Aires. Av. San Martin 4453, Buenos Aires, Argentina. maseda@ifeva.edu.ar
Summary
Micropropagation offers a viable solution for reestablishing endangered Lycium chilense in Patagonia. Subculturing microcuttings significantly improved rooting success, aiding conservation efforts for this rare shrub.
Area of Science:
- Botany
- Plant Science
- Conservation Biology
Background:
- Lycium chilense, a Patagonian shrub, is endangered by overgrazing.
- Seed germination is limited by low propagule density and specific requirements.
- In vitro propagation is crucial for reestablishing this species in degraded habitats.
Purpose of the Study:
- To develop an effective in vitro propagation protocol for Lycium chilense.
- To support the reestablishment of Lycium chilense in degraded Patagonian steppe areas.
Main Methods:
- Seeds from two provenances (varying ploidy) were used.
- Defoliated microcuttings were cultured on various media in a growth chamber.
- The impact of subculturing on rooting was evaluated.
Main Results:
- Rooting success was higher in media without hormones.
- Subculturing significantly increased rooting percentage and reduced rooting time.
- In vitro leaves showed altered morphology and reduced stomatal functionality compared to acclimated leaves.
Conclusions:
- Microcutting culture, especially with subculturing, is a feasible method for Lycium chilense repopulation.
- This protocol can aid in the conservation and restoration of Lycium chilense in its native Patagonian habitat.
