FIZICS: fluorescent imaging zone identification system, a novel macro imaging system

Area of Science:

• Biological assay development
• Imaging technology
• Biotechnology

Background:

• Advancements in biological assay development necessitate improved imaging technologies.
• Existing commercial systems lack the capability for fluorescent macroimaging of large agar plates with discrete wavelength selection.

Purpose of the Study:

• To address the limitations of current commercial imaging systems.
• To design and construct a custom imaging system for macroimaging biological samples.

Main Methods:

• A custom imaging system was designed and constructed.
• The system was engineered to capture both white light and fluorescent images.
• It accommodates agar plates from NUNC Omni tray (96-well footprint, 128 x 85 mm) to NUNC Bio Assay Dish (245 x 245 mm).

Main Results:

• The custom imaging system successfully provides fluorescent macroimaging capabilities.
• It supports imaging areas up to 245 x 245 mm.
• The system allows for discrete wavelength selection within the visible light spectrum.

Conclusions:

• The developed custom imaging system meets the technological requirements for advanced biological assay development.
• This system offers a viable solution for researchers needing to fluorescently image large agar plates with wavelength specificity.