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Usefulness of some primary anti-human antibodies in immunohistochemistry in guinea pig

H Gendek-Kubiak1, E G Gendek

  • 1Department of Cytophysiology, Histology and Embryology, Medical University of Lódź, Poland. h_kubiak@hotmail.com

Roczniki Akademii Medycznej W Bialymstoku (1995)
|January 11, 2005
PubMed

The commercially available antibodies for immunohistochemical purposes, though numerous and diversified, are mostly designed to detect human antigens. This study was undertaken to evaluate the usefulness of anti-human antigen antibodies in guinea pig tissues. The subjects of our interest were mostly skin and striated muscles and it was important for us to stain vascular and neural elements and to visualize separate cell kinds, including also skin Langerhans cells and the cells, contributing to inflammatory infiltrations.

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Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...