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Updated: Aug 6, 2026
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An Orthotopic Bladder Cancer Model for Gene Delivery Studies
Published on: December 1, 2013
HER2/neu expression in bladder cancer: relationship to cell cycle kinetics
Sanaa Eissa1, Heba S Ali, A H Al Tonsi
1Oncology Diagnostic Unit, Biochemistry Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Sanaa_eissa@hotmail.com
Clinical Biochemistry
|January 12, 2005
Summary
Human epidermal growth factor receptor 2 (HER2/neu) is overexpressed in bladder cancer and can be reliably measured by enzyme immunoassay (EIA). Quantitative HER2/neu assessment aids in predicting patient outcomes and guiding treatment decisions.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- HER2/neu overexpression is common in bladder cancer, potentially driving aberrant cell proliferation.
- This study investigates the prognostic significance of HER2/neu expression in bladder tumors.
Purpose of the Study:
- To quantitatively assess HER2/neu expression using enzyme immunoassay (EIA).
- To evaluate the prognostic value of HER2/neu in differentiating tumor proliferation rates.
- To correlate HER2/neu expression with established prognostic markers.
Main Methods:
- Enzyme immunoassay (EIA), Western blot (WB), and flow cytometry (FCM) were used to analyze HER2/neu expression.
- Tissue samples from patients with benign bladder lesions, bilharzial bladder cancer, and nonbilharzial bladder cancer were analyzed.
- HER2/neu expression was correlated with ploidy, synthetic phase fraction (SPF), DNA index (DI), lymph node status, stage, and grade.
Main Results:
- HER2/neu was significantly overexpressed in malignant bladder tumors compared to benign and normal tissues (P < 0.001).
- HER2/neu expression showed significant correlations with ploidy (P = 0.001), SPF (P = 0.012), and DNA index (P = 0.002).
- HER2/neu was significantly associated with lymph node status (P = 0.02) but not stage or grade.
Conclusions:
- Enzyme immunoassay (EIA) provides a reliable method for measuring HER2/neu expression, confirmed by Western blot (WB).
- Quantitative HER2/neu assessment, combined with proliferation markers like SPF, DI, and ploidy, can assist in patient selection for aggressive treatment and outcome prediction.
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