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In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
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Evaluation of a West Nile virus immunoglobulin A capture enzyme-linked immunosorbent assay

Harry E Prince1, Mary Lapé-Nixon

  • 1Focus Technologies, 5785 Corporate Ave., Cypress, CA 90630, USA. hprince@focustechnologies.com

Clinical and Diagnostic Laboratory Immunology
|January 12, 2005
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.