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[Determination of baicalin in Xiaoyanling Decoction by high-performance liquid chromatography]
Jun-Yi Jian1, Xiang Li, Rong-Li Qiu
1School of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China. jiang14031@sina.com
Objective:
To establish a high-performance liquid chromatography (HPLC) method for the determination of baicalin in Xiaoyanling Decoction.
Methods:
HPLC method was used for determination. A Kromosil C18 column was used. The mobile phase consisted of MeOH-acid water (H(2)O:H(3)PO(4)=53:0.2) 40:60, and the detective wavelength was 277 nm.
Results:
The content of baicalin in each Xiaoyanling Decoction sample was not less than 0.40 mg/ml (RSD<5%).
Conclusion:
The content of baicalin in Xiaoyanling Decoction is high and stable, and it can be used as the quality standard for Xiaoyanling Decoction.
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