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A new two-chip concept for continuous measurements on PMMA-microchips.
Oliver Vogt1, Markus Pfister, Ulrich Marggraf
1Institute for Analytical Sciences, P.O. Box 101352, D-44013 Dortmund, Germany. jacob@ansci.de
Lab on a Chip
|January 27, 2005
Summary
A novel polymethylmethacrylate capillary electrophoresis chip system enables continuous, automated microchip analyses. This two-chip design offers stable, reproducible measurements of small ions with minimal user intervention.
Area of Science:
- Analytical Chemistry
- Microfluidics
- Separation Science
Background:
- Continuous analysis on microchips is challenging due to stability and reproducibility issues.
- Existing microchip systems often require frequent refilling, cleaning, or chip replacement.
Purpose of the Study:
- To present a new concept for continuous measurements on microchips.
- To develop a stable and reproducible two-chip system for long-term analyses.
Main Methods:
- A polymethylmethacrylate (PMMA) based capillary electrophoresis chip with integrated conductivity detection was combined with a fluid handling and electrical connection chip.
- The system utilized a combination of electrokinetic and hydrodynamic flows.
- Characterization focused on stability and reproducibility of separation and detection of small ions.
Main Results:
- The two-chip system achieved long-term continuous stable analyses.
- Good reproducibility of migration time (<1% RSD) and peak heights (3% RSD) for analytes was demonstrated.
- The system allowed automated analysis without the need for refilling or cleaning.
Conclusions:
- The presented two-chip system offers a robust solution for continuous microchip-based analyses.
- It simplifies handling and automation, reducing the need for manual intervention.
- This approach enhances the practicality of microchip electrophoresis for routine measurements.