Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Selective intracellular lactate invisibility in Enterococcus faecalis.

P D Hockings1, M R Bendall, P J Rogers

  • 1Division of Science and Technology, Griffith University, Nathan, Australia.

Magnetic Resonance in Medicine
|April 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Obese and overweight individuals are less sensitive to information about meal times in portion-size judgements.

International journal of obesity (2005)·2017
Same author

Imaging atherosclerosis in rheumatoid arthritis: evidence for increased prevalence, altered phenotype and a link between systemic and localised plaque inflammation.

Scientific reports·2017
Same author

Parental beliefs about portion size, not children's own beliefs, predict child BMI.

Pediatric obesity·2017
Same author

Does low-energy sweetener consumption affect energy intake and body weight? A systematic review, including meta-analyses, of the evidence from human and animal studies.

International journal of obesity (2005)·2015
Same author

Chronic treatment with a tryptophan-rich protein hydrolysate improves emotional processing, mental energy levels and reaction time in middle-aged women.

The British journal of nutrition·2015
Same author

Transcriptomic and biochemical evidence for the role of lysine biosynthesis against linoleic acid hydroperoxide-induced stress in Saccharomyces cerevisiae.

Free radical research·2014
Same journal

Cartesian MPnRAGE for Efficient Simultaneous Multi-Contrast and Quantitative Relaxometry Imaging.

Magnetic resonance in medicine·2026
Same journal

Deep Learning-Based Dynamic Segmentation of the Left Atrium in 4D Flow MRI.

Magnetic resonance in medicine·2026
Same journal

Feasibility and SNR Performance of Hyperpolarized <sup>129</sup>Xe Gas Exchange Imaging Using a Balanced SSFP Sequence.

Magnetic resonance in medicine·2026
Same journal

Multi-Contrast Human Brain CEST MRI at 11.7 T: First In Vivo Demonstration.

Magnetic resonance in medicine·2026
Same journal

Suppression of Oscillation and Ghosting in RF-Spoiled Gradient-Echo-Based Dynamic Imaging.

Magnetic resonance in medicine·2026
Same journal

A Simple, Dynamic Geometric Phantom for MRI and CT Reconstruction Pipelines: Beyond Shepp-Logan.

Magnetic resonance in medicine·2026
See all related articles

Nuclear Magnetic Resonance (NMR) can track bacterial lactate gradients by analyzing T2 relaxation differences. This study reveals three distinct lactate pools, impacting in vivo spectroscopy techniques.

Area of Science:

  • Biophysics
  • Cellular Metabolism
  • Biochemistry

Background:

  • Lactate is a key metabolite in bacterial energy production.
  • Understanding lactate transport is crucial for cellular function.
  • In vivo spectroscopy relies on accurate metabolite quantification.

Purpose of the Study:

  • To demonstrate how Hahn T2 relaxation differences in 1H NMR signals can monitor lactate gradients.
  • To investigate lactate transport mechanisms in bacterial cells.
  • To identify distinct lactate pools within bacterial cells.

Main Methods:

  • Utilized 1H NMR spectroscopy to analyze lactate signals.
  • Measured Hahn T2 relaxation times for cytosolic and extracellular lactate.
  • Investigated spectral editing techniques for in vivo spectroscopy.

Related Experiment Videos

Main Results:

  • Hahn T2 relaxation differences effectively monitor lactate concentration gradients.
  • Identified at least three lactate pools with varying NMR visibility.
  • Observed implications for spectral editing techniques in bacterial spectroscopy.

Conclusions:

  • NMR-based T2 relaxation is a valuable tool for studying bacterial lactate dynamics.
  • The presence of multiple lactate pools necessitates careful consideration in spectroscopic analysis.
  • Findings impact the interpretation of in vivo NMR spectroscopy data for bacterial studies.