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U2 small nuclear RNA localization and expression during bovine preimplantation development.
A J Watson1, K E Wiemer, M Arcellana-Panlilio
1Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Molecular Reproduction and Development
|April 1, 1992
Summary
U2 small nuclear RNA (snRNA) and U snRNPs are found in bovine oocytes and embryos. Their levels remain constant until the late morula/early blastocyst stage, when U2 snRNA significantly increases.
Area of Science:
- Reproductive Biology
- Molecular Biology
- Developmental Biology
Background:
- U2 small nuclear RNA (snRNA) and U snRNPs are crucial for splicing.
- Their role during early mammalian development is not fully understood.
Purpose of the Study:
- To investigate the localization and quantity of U2 snRNA and U snRNPs during bovine preimplantation development.
Main Methods:
- In situ hybridization and immunofluorescence for localization.
- Northern blotting to quantify U2 snRNA levels.
- Utilized in vitro maturation, fertilization, and co-culture systems.
Main Results:
- U2 snRNA and U snRNPs were observed in the germinal vesicle, ooplasm, and pronuclei of oocytes and early embryos.
- Post-fertilization, they localized to the interphase nucleus of blastomeres.
- U2 snRNA levels remained stable until the late morula/early blastocyst stage, then increased 4.4-fold.
Conclusions:
- A maternal store of snRNAs is maintained in bovine preimplantation embryos.
- These molecules are dynamically regulated during early development.