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A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry
Published on: August 21, 2009
Determination of micelle mass by electrospray ionization mass spectrometry
Daisuke Nohara1, Takatomo Kajiura, Koji Takeda
1Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu 501-1193, Japan. danohara@biomol.gifu-u.ac.jp
Abstract:
By electrospray ionization (ESI) mass spectrometry, micelle solutions of sodium cholate were investigated in detail in the presence and absence of ethanol. The average aggregation number could be evaluated from the spectra acquired under conditions where soft collisions adequate to measure the micelle solution were induced, and the value agreed well with that obtained previously by other methods. From the dependence on ethanol content, it was also found that the average aggregation number in aqueous solution without organic solvent could be reliably estimated. The ESI method proved to be a useful tool for determining the micelle mass in the original aqueous phase.
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