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Updated: Jul 16, 2026
Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
Published on: May 29, 2010
Microarray analysis of genes that respond to gamma-irradiation in Arabidopsis
Toshifumi Nagata1, Hitomi Yamada, Zeji Du
1National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Abstract:
To reveal the signal transduction mechanism of the response to stress in the form of active oxygen species, we used a microarray system to analyze gene expression patterns 2 or 24 h after gamma-irradiation of Arabidopsis. gamma-Irradiation induces several signal transduction and metabolite genes. By analysis of cis-elements located on the promoter region of the gamma-responsive genes, we have also found several cis-elements related to various signal transduction systems. We also analyzed the pleiotropic mutant ttg1-1, which has a dramatically altered physiological response to gamma-irradiation. By comparing the gene expression patterns of wild-type (Ler) and ttg1-1 mutant plants after gamma-irradiation, we identified various TTG1-regulated gamma-response genes. Analysis of the cis-elements in the promoter region of the gamma-responsive genes also revealed that the many transcription factors interacting with TTG1 protein (WD40 protein) are related to the gamma-responsive gene expression.
