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Updated: Sep 9, 2026
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
Thiol-Ene Click-Derived Porous-Layer Open-Tubular Column for Online Purification and Multiplex LC-MS/MS Analysis of
Enrico Taglioni1,2, Andrea Cerrato1,2, Aldo Laganà1,2
1Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185Rome, Italy.
Abstract:
A thiol-ene-based porous stationary phase (PLOT-type coating) was developed for online sample cleanup and enrichment coupled to LC-MS/MS for the simultaneous determination of structurally diverse neurotransmitters in biological matrices. The resulting porous thiol-ene functionalized capillary column combines the inherently high permeability of the open-tubular architecture with tailored surface functionality, enabling direct injection and efficient handling of highly polar, low-abundance analytes without derivatization. The method allows multiplexed quantification of serotonin, dopamine, glutamic acid, γ-aminobutyric acid, and reduced glutathione in plasma, cell samples, and brain tissue within a simplified analytical workflow. Compared to conventional off-line protocols, the proposed approach minimizes sample preparation while maintaining high sensitivity and reproducibility, with recoveries ranging from 86% to 100% and matrix effects within 90-104%. The platform demonstrates reliable analytical performance across different biological matrices and is compatible with routine LC-MS/MS instrumentation. Overall, this work establishes the thiol-ene-porous capillary as a viable strategy for integrated sample processing in bioanalysis and expands its application to polar metabolite profiling in complex systems.
