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Updated: Aug 19, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Comparison of microemulsion electrokinetic chromatography and micellar electrokinetic chromatography as methods for
Hsi-Ya Huang1, Chen-Wen Chiu1, Yen-Chen Chen1
1Department of Chemistry and Center for Nanotechnology at CYCU, Chung Yuan Christian University, Chung-Li, Taiwan, R.O.C.
Abstract:
Microemulsion electrokinetic chromatography (MEEKC) and micellar electrokinetic chromatograpy (MEKC) were compared for their abilities to separate and detect ten similar benzophenones, which are commonly used as UV filters in various plastic and cosmetic products. Sodium dodecyl sulfate (SDS) concentration and column temperature rarely affected separation resolution for MEEKC, but separation of benzophenones could be improved by changing the SDS concentration and column temperature for MEKC. Buffer pH and ethanol (organic modifier) were found to markedly influence the separation selectivity for both MEEKC and MEKC systems. In addition, a higher electric voltage improved the separation efficiency without a noticeable reduction in separation resolution for MEEKC, whereas it caused a poor separation resolution for the MEKC system.
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