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Updated: Aug 19, 2026
A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Published on: March 15, 2016
[Expression of VEGF-C and VEGFR-3 in human laryngeal squamous cell carcinomas and cervical metastatic lymph node
Zhongliang Wang1, Yao Chen, Chuanyu Liang
1Department of Human Anatomy, West China of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China.
Objective:
To study the gene expression of vascular endothelial growth factor C (VEGF-C) and vascular endothelial growth factor receptor-3 (VEGFR-3) in laryngeal squamous carcinoma and their relation with clinic pathologic features.
Method:
VEGF-C and VEGFR-3 gene expression in 30 cases of normal laryngeal mucosa tissue (NLM), primary laryngeal carcinoma (PLC) and cervical lymph node (CLN) were examined by reverse transcription polymerase chain reaction (RT-PCR).
Result:
The gene expression of VEGF-C and VEGFR-3 are different among normal laryngeal mucosa tissue, primary laryngeal carcinoma and cervical lymph node in the same patient. In primary laryngeal carcinoma, expression of VEGF-C, VEGFR-3 were significantly higher in lymph node positive group than in lymph node negative group and associated with histological grade of differentiation; The gene expression of VEGF-C, VEGFR-3 were not associated with age, sex, site, T stage.
Conclusion:
VEGF-C, VEGFR-3 have prognostic significance in laryngeal carcinoma.
