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Updated: Aug 19, 2026

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus
Published on: August 25, 2017
[Malignant transformation of a human oral squamous cell carcinoma cell strain OSC-19 by EBV encoded small RNAs]
Qingchun Ren1, Tengbo Zuo, Hongguang Lü
1Department of Otolaryngology, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China.
Objective:
To observe the growth characteristics of the EBERs-transfected OSC (oral squamous carcinoma)-19 cells, through generated overexpression of EBERs in OSC-19 cells.
Method:
EBERs fragment were amplified by PCR from EBV-positive NPC-KT cell DNA. Using molecular biologic technique, we constructed the EBERs-expressing plasmids, which contained, 4 or 10 tandem repeats of the EBERs. The OSC-19 cells were transfected with those plasmids, respectively. We demenstrated the overexpression of EBERs in EBERs-transfected OSC-19 cells by Northern Blot analysis, and compared the growth characteristics in culture and colony formation in soft agar of Hygr- and EBERs-transfected OSC-19 cells.
Result:
10EBERs-transfected OSC-19 cells lost cell-to-cell contacts and acquired long spindle-shape morphology in culture and formed colonies in soft agar. However, parental and Hygr-transfected OSC-19 cells scarcely did.
Conclusion:
In this study, we established 10 EBERs-transfected OSC-19 cells, which overexpressing the EBERs and showed that it has a scattering activity in culture and a role in anchorage-independent growth in soft agar. These results indicate that EBERs has a role of malignant transformation in OSC-19 cells. However, the 10 EBERs-transfected OSC-19 cell provides a useful epithelial model for studying the function of EBERs.
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