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Updated: Jul 31, 2026
High-Throughput Analysis of Non-Photochemical Quenching in Crops Using Pulse Amplitude Modulated Chlorophyll Fluorometry
Published on: July 6, 2022
Effect of bio-optical parameter variability on the remote estimation of chlorophyll-a concentration in turbid
Giorgio Dall'Olmo1, Anatoly A Gitelson
1Center for Advanced Land Management Information Technologies, School of Natural Resources, University of Nebraska-Lincoln, 113 Nebraska Hall, Lincoln, Nebraska 68588-0517, USA. gdall@calmit.unl.edu
Abstract:
The analytical development and underlying hypothesis of a three-band algorithm for estimating chlorophyll-a concentration ([Chla]) in turbid productive waters are presented. The sensitivity of the algorithm to the spectral location of the bands used is analyzed. A large set of experimental observations ([Chla] varied between 4 and 217 mg m(-3) and turbidity between 2 and 78 nephelometric turbidity units) was used to calibrate and validate the algorithm. It was found that the variability of the chlorophyll-a fluorescence quantum yield and of the chlorophyll-a specific absorption coefficient can reduce considerably the accuracy of remote predictions of [Chla]. Instead of parameterizing these interferences, their effects were minimized by tuning the spectral regions used in the algorithm. This allowed us to predict [Chla] with a relative root-mean-square error of less than 30%.
