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Related Experiment Videos

Volume cytometry: microfluidic sensor for high-throughput screening in real time.

Daniel A Ateya1, Frederick Sachs, Philip A Gottlieb

  • 1Bio-MEMS and Bio-Materials Laboratory, Department of Mechanical and Aerospace Engineering, SUNY-Buffalo, Buffalo, New York 14260, USA.

Analytical Chemistry
|March 1, 2005
PubMed
Summary
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A novel microchip measures cell volume in real time, enabling rapid antibiotic sensitivity testing and discovery of new compounds. This technology offers a faster, more efficient approach to cell-based screening and diagnostics.

Area of Science:

  • Biophysics
  • Cell Biology
  • Microfluidics

Background:

  • Cell volume regulation is a fundamental metabolic indicator.
  • Conventional cell volume measurement methods are time-consuming and complex.
  • Cell volume has been underutilized in cell-based screening assays.

Purpose of the Study:

  • To develop a microfabricated chip for real-time cell volume measurement.
  • To demonstrate the chip's application in antibiotic sensitivity testing and natural product screening.
  • To overcome limitations of existing cell volume measurement techniques.

Main Methods:

  • Development of a microfluidic chip for real-time cell volume analysis.
  • Application of the chip to adherent and suspended cells, including membrane-bound organelles.

Related Experiment Videos

  • Testing the chip's sensitivity with cultured astrocytes and bacterial strains.

    • Main Results:

    • The chip measures cell volume in real time with high resolution.
    • Detected volume changes in astrocytes responding to <1mOsm stimuli.
    • Determined antibiotic sensitivity of E. coli strains in under 10 minutes.
    • Identified a spider venom peptide inhibiting eukaryotic volume regulation at 100pM.

    Conclusions:

    • The developed microchip offers a rapid, high-resolution method for cell volume measurement.
    • This technology facilitates quick antibiotic sensitivity testing and drug discovery.
    • The inexpensive, reusable silicon chip technology is scalable for broader applications.