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The death-inducing effect and genomic instability.

Shruti Nagar1, William F Morgan

  • 1Radiation Oncology Research Laboratory, University of Maryland, Baltimore, MD 21201-1559, USA.

Radiation Research
|March 1, 2005
PubMed
Summary
This summary is machine-generated.

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Ionizing radiation causes genomic instability in cells. Secreted factors from irradiated cells can harm stable cells but also induce heritable instability in survivors, perpetuating this effect.

Area of Science:

  • Cell biology
  • Radiation biology
  • Genetics

Background:

  • Ionizing radiation exposure can cause non-targeted effects in unirradiated cells.
  • Genomic instability is a potential early step in radiation carcinogenesis.
  • Secreted factors from irradiated cells are implicated in inducing genomic instability.

Purpose of the Study:

  • To investigate the role of secreted factors from chromosomally unstable cells in inducing heritable changes.
  • To understand the mechanism of perpetuating genomic instability through cell-to-cell communication.

Main Methods:

  • Exposure of GM10115 cells to medium from chromosomally unstable clones.
  • Clonal expansion of surviving cells.
  • Cytogenetic analysis, including micronucleus and HPRT mutation frequency assays.
Keywords:
NASA Discipline Radiation HealthNon-NASA Center

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Main Results:

  • Medium from unstable clones was cytotoxic to most stable cells.
  • A subset of surviving cells exhibited delayed chromosomal instability.
  • Increased micronucleus and HPRT mutation frequencies were observed in some survivor clones.

Conclusions:

  • Genomically unstable cells secrete factors that are cytotoxic to stable cells.
  • These factors can induce heritable genomic instability in surviving cells.
  • This suggests a mechanism for the perpetuation of instability via secreted cytotoxic factors.