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Updated: Aug 19, 2026

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
Published on: December 26, 2011
[Substrate specificity and action mechanism of glycosidases]
Abstract:
Result of author's research and data from literature have been generalized with respect to hydrolase and transferase activity of glycosidases: alpha-galactosidase and alpha-N-acetylgalactosaminidase--the enzymes which catalyse hydrolysis of natural and synthetic glycosides. Broad variability of action specificity of glycosidases with respect to glycon, aglycon as well as the bond type depending on the enzyme isolation source have been shown. One can suppose that the enzyme action specificity is connected with different formation mechanisms of enzyme-substrate complexes. An idea is discussed concerning the identity of the mechanism of splitting of various glycosidic links by the studied enzymes.
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