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[Technical problems in microsurgical processing of preserved donor tissue].
1Universitäts-Augenklinik.
Summary
Microsurgical processing of preserved donor tissue for keratoplasties remains challenging. This study presents advantages of rotortrephine cutting and optimal tissue examination and storage methods.
Area of Science:
- Ophthalmology
- Microsurgery
- Tissue Engineering
Context:
- Challenges in microsurgical processing of long-term preserved donor tissue for lamellar and perforating keratoplasties.
- Need for improved techniques in preparing corneal tissue for transplantation.
Purpose:
- To present the advantages of using rotortrephines compared to manual trephines for cutting donor tissue.
- To explain optimal methods for examining and storing donor tissue.
- To introduce novel instrumentation for corneal tissue processing.
Summary:
- Donor tissue preparation for keratoplasty, particularly lamellar and perforating keratoplasties, presents challenges with preserved tissues.
- The study highlights benefits of using rotortrephines over manual trephines for precise donor tissue cutting.
- Optimal examination and storage techniques for donor tissue are detailed.
- Novel instruments including a lamellar microkeratome, an artificial anterior chamber for corneoscleral discs, and a refractive workbench for non-freeze modification are demonstrated.
Impact:
- Potential to improve the quality and outcomes of corneal transplantation procedures.
- Advancement in microsurgical techniques for ophthalmic tissue processing.
- Facilitation of better donor tissue utilization and preservation for keratoplasty.