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Updated: Aug 18, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Analysis of diltiazem and its related substances by HPLC and HPLC/MS
M G Quaglia1, E Donati, S Fanali
1Dipartimento Studi Farmaceutici, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, Rome 00185, Italy. mariagiovanna.quaglia@uniroma1.it
Abstract:
Diltiazem (DTZ) is an optically active calcium channel blocker having a benzodiazepine structure. The drug used in therapy is (+)-cis-diltiazem with configuration (2S,3S). To describe the analytical profile of DTZ different stationary phases (RP-18, RP-8, monolithic support) were tested. The best separation of DTZ from A, B, E and F was obtained using as stationary phase a RP-8 or a monolithic RP-18. The characterization of impurities was carried out using two analytical systems, HPLC and HPLC/MS.
