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Candida albicans adherence to endothelial cells.

C L Mayer¹, S G Filler, J E Edwards

¹Department of Medicine, Harbor/UCLA Medical Center, Torrance 90509.

Microvascular Research

|March 11, 1992

Summary

A new microtiter plate system accurately quantifies microbial adherence to endothelial cells. This method simplifies studying molecular mechanisms of infection by avoiding difficult washing steps.

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Area of Science:

Microbiology
Cell Biology
Biotechnology

Background:

Understanding microbial adherence to endothelial cells is crucial for elucidating infection mechanisms.
Current methods using expensive reagents limit the study of these molecular interactions.
A need exists for a cost-effective and reliable microsystem to quantify adherence.

Purpose of the Study:

To develop and validate a 96-well microtiter plate system for quantifying microbial adherence to human umbilical vein endothelial cells.
To overcome challenges of coadherence and non-specific binding in adherence assays.
To provide a simplified and reproducible method for studying adherence mechanisms.

Main Methods:

Utilized L-[35S]methionine-labeled Candida albicans and human umbilical vein endothelial cells in a 96-well microtiter plate system.
Grew endothelial cells on a collagen matrix in detachable microwells for easy assessment.
Quantified adherence by measuring radioactive counts of the entire well, avoiding removal of adherent microorganisms.

Main Results:

The developed microsystem accurately quantified Candida albicans adherence to endothelial cells.
The method effectively minimized variability by expressing adherence as a percentage of total organisms added.
Detachable microwells and direct radioactive counting eliminated the need for washing steps, simplifying the assay.

Conclusions:

The 96-well microtiter plate system offers a sensitive, reproducible, and simplified method for studying microbial adherence to endothelial cells.
This microsystem facilitates research into the molecular mechanisms of microbial-endothelial cell interactions.
The technique is valuable for reducing costs and improving the efficiency of adherence studies.

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