Related Experiment Video
Updated: Aug 18, 2026
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Published on: January 24, 2017
[Proteome analysis of nuclear matrix proteins during arsenic trioxide induced apoptosis in K562 cells]
Zi-hui Wang1, Ding Yu, Jie Zheng
1Department of Pathology, Peking University Shenzhen Hospital, Shenzhen 518036, China.
Objective:
To investigate arsenic trioxide (As(2)O(3))-target interactions at the level of nuclear matrix (NM) in chronic myelogenous leukemia cell line K562 by proteomics.
Methods:
DNA fragmentation analysis was used for As(2)O(3) induced apoptosis of K562 cells. The nuclear matrix proteins were analyzed by high-resolution two-dimensional gel electrophoresis and computer-assisted image analysis.
Results:
While more than 200 protein spots were shared among the nuclear matrices, about 18 distinct spots were found characteristic of As(2)O(3) treated cells. Onset of mass mange apoptosis, and the profiling of nuclear matrix proteins had been alternated and it was a more sensitive indicator than nucleosomal DNA fragmentation against As(2)O(3) treatment.
Conclusion:
As(2)O(3) induced apoptosis in K562 cells in a dose-time-dependent manner. As(2)O(3) might be clinically useful in treatment of chronic myelogenous leukemia and the changes of nuclear matrix proteins in the treated cells can be used as a useful indicator for the treatment.
