Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-throughput multiplex single-nucleotide polymorphism analysis for red cell and platelet antigen genotypes.

G A Denomme1, M Van Oene

  • 1Research and Development, Canadian Blood Services, the Toronto Centre, Toronto, Ontario, Canada. greg.denomme@bloodservices.ca

Transfusion
|April 26, 2005
PubMed
Summary

Genotyping blood donors using single-nucleotide polymorphisms (SNPs) offers a cost-effective alternative to antibody-based phenotyping for red cell and platelet (PLT) antigens. This SNP assay accurately identifies key blood group and HPA genotypes, improving transfusion safety.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of cryopreservation on a rare McLeod donor red blood cell concentrate.

Immunohematology·2021
Same author

Unusual serological findings associated with ceftriaxone-induced immune hemolytic anemia in a child with disseminated low-grade glioma.

Pediatric blood & cancer·2016
Same author

Consortium for Blood Group Genes (CBGG): 2009 report.

Immunohematology·2010
Same author

Consortium for Blood Group Genes (CBGG): 2008 report.

Immunohematology·2009
Same author

Maternal ABO-mismatched blood for intrauterine transfusion of severe hemolytic disease of the newborn due to anti-Rh17.

Transfusion·2004
Same author

ABO sequence analysis in a family with weak expression of blood group B.

Transfusion·2004

Area of Science:

  • Immunology
  • Genetics
  • Transfusion Medicine

Background:

  • Alloimmunization in transfusion recipients necessitates antigen-negative blood products.
  • Traditional antibody-based phenotyping is costly and resource-intensive.
  • Genomic DNA screening using single-nucleotide polymorphisms (SNPs) presents an alternative phenotyping strategy.

Purpose of the Study:

  • To develop and validate a multiplex polymerase chain reaction (PCR)-oligonucleotide extension assay for SNP genotyping.
  • To assess the accuracy of SNP genotyping for identifying red cell and human platelet antigens (HPAs).
  • To evaluate the efficiency of a SNP-based platform for high-throughput blood donor screening.

Main Methods:

  • Developed a multiplex PCR-oligonucleotide extension assay on a SNP genotyping platform.

Related Experiment Videos

  • Analyzed 372 samples for 12 SNPs associated with blood group and HPA antigens.
  • Compared SNP genotypes with established blood group and HPA phenotypes.

    • Main Results:

    • The assay achieved 98-100% concordance for 11 of 12 tested SNPs.
    • Accurate identification of D antigen (98.6%) and specific genotypes like R(1)R(1), r"r, and HPA-1b/1b.
    • Lowest concordance (89.5%) observed for RHCE exon 5 E/e SNP analysis.

    Conclusions:

    • The SNP genotyping platform is capable of high-throughput analysis, processing thousands of samples daily.
    • Genotype data for all tested SNPs can be obtained within 36 hours.
    • This SNP-based approach offers an efficient and potentially cost-effective method for blood donor screening.