Related Experiment Video
Updated: Aug 18, 2026
Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart
Published on: September 10, 2015
[The effect of the extract from Ranunculus japonicus on [Ca2+]i inside rabbit VSMC by serologic pharmacological test]
1Department of Pharmacy, Guangdong College of Pharmacy, Guangzhou 510224.
Objective:
To observe the influence of serum containing the extract from Ranunculus japonicus (ERj) on the amount of [Ca2+]i inside vascular smooth muscle cells (VSMC).
Methods:
Serum containing ERj was prepared by serum pharmacological method. Cultured VSMCs induced by NE were treated with the serum, and the influence of it on the amount [Ca2+]i inside cells indicated with INDO-AM was determined.
Results:
The serum containing ERj could markedly decrease the increase of the amount of free [Ca2+]i induced by NE in cultured VSMC.
Conclusion:
The serum containing ERj could decrease the intracellular [Ca2+]i.
