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Updated: Aug 14, 2026

Determination of the Gas-phase Acidities of Oligopeptides
Published on: June 24, 2013
Characterization of quaternary ammonium oligomers by paired-ion reversed-phase liquid chromatography-mass
A J Bourque1, S P Clark, A Kloss
1Analytical Research and Development, Genzyme Drug Discovery and Development, Genzyme Corporation, 153 Second Avenue, Waltham, Massachusetts 02451, USA.
Abstract:
The separation of novel permanently charged oligomers was studied using paired-ion reversed-phase liquid chromatography. The polyionene studied is less than 5 kDa in size, but contains three oligomer series with different end-group chemistries. The complexity of this polyionene makes development of a single-dimension separation quite challenging. Separation under critical conditions was employed to fractionate the end-group conformations and then the chain length of the oligomers in each series was confirmed by LC-MS. The oligomers were then used to optimize a single-dimension HPLC separation. Precise modulation of the hydrophobicity of the ion-pair reagent and the stationary-phase chemistry yielded very high resolution one-dimensional separations.
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