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RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
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mRNA: detection by in situ and northern hybridization

Alessandra P Princivalle1, Rachel M C Parker, Terri J Dover

  • 1Cellular and Molecular Neuropharmacology Research Group, Division of Neuroscience, Department of Pharmacology, The Medical School, University of Birmingham, Birmingham, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 4, 2005
PubMed
Abstract

No abstract available in PubMed .

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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