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C-CAM (Cell-CAM 105) is a calmodulin binding protein
I Blikstad1, T Wikström, M Aurivillius
1Department of Medical and Physiological Chemistry, University of Uppsala.
FEBS Letters
|May 4, 1992
Summary
Cell-CAM 105, a cell adhesion molecule, specifically binds to calmodulin. This interaction occurs at the cytoplasmic domain of Cell-CAM 105 in a calcium-dependent manner.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Cell-CAM 105 (also known as C-CAM) is a transmembrane cell adhesion molecule.
- It belongs to the immunoglobulin superfamily and mediates intercellular adhesion in rat hepatocytes.
- C-CAM is found in various isoforms across epithelia, endothelia, and leukocytes.
Purpose of the Study:
- To investigate the interaction between purified liver C-CAM and calmodulin.
- To determine the binding site of calmodulin on C-CAM.
- To identify C-CAM as a calmodulin-binding protein in liver membranes.
Main Methods:
- Dot-blot assay using 125I-labeled C-CAM.
- Gel overlay assay using 125I-labeled calmodulin.
- Protease digestion to generate peptides for binding analysis.
- Analysis of whole liver membranes.
Main Results:
- Purified liver C-CAM specifically interacts with calmodulin.
- Calmodulin binds to the cytoplasmic domain of C-CAM.
- C-CAM is one of five major proteins in whole liver membranes that bind calmodulin in a calcium-dependent manner.
Conclusions:
- Cell-CAM 105 interacts directly with calmodulin.
- The binding site is located within the cytoplasmic domain of Cell-CAM 105.
- This interaction is calcium-dependent, suggesting a role in cellular signaling or regulation.