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Updated: Jul 15, 2026
Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Monosaccharide composition analysis of glycoproteins by isotope-tag method and capillary LC/ESI-MS
Jin Yuan1, Nana Kawasaki, Noritaka Hashii
1Gene Engineering Department, Chengdu Institute of Biological Products, Chengdu 610063, China. yuanjinv@hotmail.com
Aim:
To develop a rapid and sensitive method for monosaccharide composition analysis.
Methods:
Glycoprotein was first hydrolyzed to monosaccharides, which were subsequently reacetylated (amino monosaccharides), tagged with 2-aminopyridine and then separated and monitored in selected ion mode by CapGCC-LC/MS. The use of tetradeuterium labeled-aminopyridyl-monosaccharides prepared by tagging monosacahrides with hexadeuterium labeled 2-aminopyridine as internal standards improved the linearity and reproducibility in quantification.
Results:
This method was successfully applied to monosaccharide composition analysis of model glycoproteins, fetuin and erythropoietin down to 1 pmol monosaccharides.
Conclusion:
This method has been shown to be highly sensitive and is applicable to monosaccharide composition analysis of glycoproteins.
