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Large-scale imaging of cortical network activity with calcium indicators
Yuji Ikegaya1, Morgane Le Bon-Jego, Rafael Yuste
1Department of Biological Sciences, Columbia University, 1002 Fairchild Center, M.C. 2435 New York, NY 10027, USA. ikegaya@tk.air.jp
Neuroscience Research
|May 17, 2005
Summary
Bulk loading of calcium indicators allows detailed reconstruction of cortical network activity. This study details methods for bulk loading AM dyes for high-resolution imaging using spinning disk confocal microscopy.
Area of Science:
- Neuroscience
- Cell Biology
- Microscopy
Background:
- Bulk loading of calcium indicators offers a method for studying neuronal activity.
- Reconstructing cortical network activity requires high-resolution imaging techniques.
Purpose of the Study:
- To describe detailed methods for bulk loading AM dyes.
- To optimize these methods for imaging with spinning disk confocal microscopy.
Main Methods:
- Bulk loading of AM dyes into cortical networks.
- Imaging using a spinning disk confocal microscope.
Main Results:
- Successful application of bulk loading technique for calcium indicator delivery.
- High-resolution imaging of cortical networks achieved.
Conclusions:
- Bulk loading of AM dyes is a viable method for studying cortical networks.
- The described methods facilitate single-cell resolution imaging of neuronal activity.