Related Experiment Videos
Paraoxonase-1 activity modulates endothelial function in patients with peripheral arterial disease
Leonella Pasqualini1, Claudio Cortese, Simona Marchesi
1Unit of Internal Medicine, Angiology and Arteriosclerosis, Department of Clinical and Experimental Medicine, University of Perugia, Perugia, Italy. lpasqua@unipg.it
Atherosclerosis
|May 19, 2005
Summary
Human serum paraoxonase-1 (PON1) activity, influenced by its Q192R polymorphism, directly impacts peripheral endothelial function. Higher PON1 activity is linked to improved brachial artery vasodilation in patients with peripheral arterial disease.
Area of Science:
- Cardiovascular Research
- Human Genetics
- Biochemistry
Background:
- Human serum paraoxonase-1 (PON1) is implicated in the beneficial vascular effects of high-density lipoproteins, primarily by inhibiting low-density lipoprotein oxidation.
- Endothelial dysfunction, an early marker of atherosclerosis, signifies an artery's reduced ability to dilate.
Purpose of the Study:
- To investigate the impact of PON1 Q192R polymorphism and serum activity on peripheral endothelial function.
- To assess endothelial function using brachial-artery flow-mediated vasodilation (FMV) in patients with peripheral arterial disease.
Main Methods:
- Study included 37 patients diagnosed with peripheral arterial disease.
- PON1 activity and Q192R polymorphism were analyzed.
- Brachial-artery flow-mediated vasodilation (FMV) was measured to evaluate endothelial function.
Main Results:
- Patients with the R allele (QR or RR genotypes) exhibited significantly higher PON1 activity and greater brachial FMV compared to those with the QQ genotype.
- A positive correlation was observed between PON1 activity and brachial FMV across the entire study population.
- Multivariate analysis identified PON1 activity, brachial artery diameter, and male sex as independent predictors of brachial FMV.
Conclusions:
- PON1 activity is a significant determinant of peripheral endothelial function.
- The findings underscore the role of PON1 in modulating vascular health, particularly in the context of atherosclerosis progression.