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Progressive elimination of microinjected trehalose during mouse embryonic development.
Ali Eroglu1, Gloria Elliott, Diane L Wright
1Institute of Molecular Medicine and Genetics, Medical College of Georgia, 1120 15th Street, CB 2803, Augusta, GA 30912, USA.
Reproductive Biomedicine Online
|May 20, 2005
Summary
Microinjected trehalose is rapidly eliminated from mouse oocytes during embryonic development. This suggests trehalose is cleaved into glucose, impacting future culture media design for cryopreservation.
Area of Science:
- Cell Biology
- Biochemistry
- Developmental Biology
Background:
- Sugars like trehalose enhance cell tolerance to freezing and drying stresses.
- The metabolic fate of intracellular trehalose in mammalian oocytes remains unclear.
Purpose of the Study:
- To investigate the intracellular fate of microinjected trehalose during mouse oocyte and early embryonic development.
- To establish a sensitive method for quantifying intracellular sugar concentrations.
Main Methods:
- Microinjection of 0.1 mol/l trehalose into mouse oocytes.
- High-performance liquid chromatography with pulsed amperometric detection (HPLC-PAD) to measure intracellular trehalose and glucose.
- Analysis throughout embryonic development stages.
Main Results:
- Trehalose was undetectable in control, non-injected oocytes.
- Intracellular trehalose levels decreased progressively during embryonic development.
- A corresponding increase in intracellular glucose was observed at the two-cell stage, indicating trehalose hydrolysis.
Conclusions:
- Microinjected trehalose is rapidly metabolized and eliminated during mouse embryonic development.
- Trehalose is likely cleaved into glucose, influencing intracellular osmolarity.
- Findings are crucial for optimizing culture media for sugar-injected oocytes and improving cryopreservation strategies.